Application Note

Characterizing cell types in tissues using digital cytometry 

An image of a hand holding up three slices of paraffin-embedded tissue on a slide

 

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An image of a hand holding up three slices of paraffin-embedded tissue on a slide
 
Typically, once a tissue sample has been frozen or formalin-fixed and paraffin-embedded, the only way to resolve its cellular composition is to use immunohistochemistry or immunofluorescence, which relies on a limited number of markers. Digital cytometry software uses tissue gene expression data to resolve diverse cell types without complicated staining procedures. 

Download this application note from Agilent to learn how pairing digital cytometry software with bulk gene expression data enables the identification of hematopoietic cell subtypes, fibroblasts, endothelial cells, and epithelial cells from fresh, frozen, or fixed tissues.

 

 

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